Bone Abstracts

Objectives: Recent studies have demonstrated that vitamin K2 (VitK2) induces apoptosis of not only osteoclasts but also rheumatoid arthritis (RA) synovial cells in vitro, while its clinical effect on disease activity of RA remains unknown.Methods: 158 female RA patients (age 62.5 years, duration of disease 14.9 years) who had not been treated with warfarin, biologics, or teriparatide were enrolled in this study. VitK2 (45 mg/day) was administered orally ...

Objective: To examine the efficacy and safety of direct administration of zoledronate acid (ZOL) on local osteoporotic lesion of ovariectomized rats.Methods: Six weeks later after ovariectomy, 16 6-month-old female S.D. rats were divided into the two groups with no differences of body weight and BMD of the proximal tibia. In the group L, 50 μl ZOL at a dose of 67 μg/kg were locally injected into the bone marrow between the two dr...

Objectives: Sox9 is a dominant but insufficient transcription factor to induce thorough primary chondrogenesis, so other factors which may induce primary chondrogenesis besides Sox9 have been assumed. The previously reported function of transcription factor Nkx3.2 is to maintain chondrogenic phenotype by suppressing Runx2, while recent studies demonstrated that mouse Nkx3.2 null mice shows severe metaphyseal dysplasia which is similar to that seen in type II collagen a1 (Col2a...

Introduction: A synthetic prostacyclin IP receptor agonist (ONO-1301) has been reported to induce the production of endogenous HGF and VEGF in fibroblasts by stimulating cAMP production.Materials and methods: In vitro; murine primary osteoblasts and cell line (ST2, MC3T3-E1, C2C12) were treated with BMP-2 (0–100 ng/ml) and ONO-1301 (0–10−6 M). ALP assay and cell proliferation assay were performed. In vivo analysis, Col...

Introduction: Indirect inhibition of the extracellular BMP antagonist network and the activation of Wnt signaling by PTH1-34 suggest the possibility of synergistic effect on bone regeneration by the co-administration of BMP and PTH1-34.Materials and methods: In a rat critical-sized femoral defect (7 mm) model, SD rats (n=32) were operated by absorbable collagen sponge containing two different BMP-2 dosage treatments; 1) 2 μg (low dose) and ...

Objective: The purpose of this study is to investigate the effects of the combined teriparatide (TPTD) and denosumab (DMAB) on both cortical and cancellous bone regeneration.Materials and methods: Bone defects were created in the diaphysis of the right femur and in the epimetaphysis of the left femur in 8-week old female C57/BL6N mice. After making bone defects, Mice were given either saline or 40 μg/kg TPTD for 20 days (5× per week) and were s...